Subcellular Distribution of DNA Origami Nanostructures

doi:10.21127/yaoyimr20190100

Medicine Research ›› 2018, Vol. 2 ›› Issue (4): 190100-.DOI: 10.21127/yaoyimr20190100

Subcellular Distribution of DNA Origami Nanostructures

Yuanjie Zhu^a, Ping Wang^{a, *}, Ting Liu^b, Yi Chen, Ruxin Zhou, Xiaodi Li, Yan Xu^b, Yinan Yan^b, Jun Zhu^b, Gang Huang^c, Dannong He^{a, b, *}

a School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology,Shanghai 200093, China;
b National Engineering Research Center for Nanotechnology, Shanghai 200241, China;
c Shanghai University of Medicine &Health Sciences, Shanghai, 201318, China;

Received:2019-01-08 Revised:2019-01-28 Online:2018-12-20 Published:2019-03-04
Contact: Email: pingw2006@163.com (P. W.), hdn_nercn@163.com (D. H.).
^†These authors contributed equally to this work.

Abstract

Abstract: As a new type of material with application prospect in drug delivery system, DNA origami nanostructures (DONs) have showed unparalleled abilities on nanoparticle drug delivery. However, the subcellular distribution of DONs after entering cells remained unclarified. Herein, we studied the phagocytic rate of U87 cells on fluorescent triangular DNA origami and Cy5 labelled Gold nanoparticles (Cy5-AuNPs) after 24 h. Atomic force microscope, confocal microscopy and immunofluorescence analysis (four organelles) were used to characterize their morphology. We found that DONs distributed in late endosome and lysosome. Meanwhile, gold nanoparticles enter the cells from early endosome to late endosome, and then exit the cells through exocytosis. The results provide us the novel insight for the design and optimization of DNA triangular origami nanostructures vehicles for drug delivery.

Key words: DNA origami, gold nanoparticles, subcelluar distribution

Yuanjie Zhu, Ping Wang, Ting Liu, Yi Chen, Ruxin Zhou, Xiaodi Li, Yan Xu, Yinan Yan, Jun Zhu, Gang Huang, Dannong He. Subcellular Distribution of DNA Origami Nanostructures[J]. Medicine Research, 2018, 2(4): 190100-.

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Subcellular Distribution of DNA Origami Nanostructures

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