Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro

doi:10.21127/yaoyimr20200010

Medicine Research ›› 2020, Vol. 4 ›› Issue (3): 200010-.DOI: 10.21127/yaoyimr20200010

所属专题： Phytomedicine

Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro

Xiaojing Shen^a,b,c and Weiwei Jiang^*,a,d,e

^a College of Science, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^bParty Committee of Organ, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^cCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^d National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Kunming, Yunnan 650201, China
e Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming, Yunnan 650201, China

收稿日期:2020-05-25 修回日期:2020-06-16 出版日期:2020-09-22 发布日期:2020-09-22
通讯作者: Email: 2015031@ynau.edu.cn (W. J.)

Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro

Xiaojing Shen^a,b,c and Weiwei Jiang^*,a,d,e

^aCollege of Science, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^bParty Committee of Organ, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^c College of Food Science and Technology, Yunnan Agricultural University, Kunming, Yunnan 650201, China
^d National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Kunming, Yunnan 650201, China
e Key Laboratory of Medicinal Plant Biology, Yunnan Agricultural University, Kunming, Yunnan 650201, China

Received:2020-05-25 Revised:2020-06-16 Online:2020-09-22 Published:2020-09-22
Contact: Email: 2015031@ynau.edu.cn (W. J.)

摘要/Abstract

摘要： In this paper, the extraction conditions of water-soluble polysaccharide from Bletilla striata were optimized by response surface methodology. With the dried Bletilla striata as raw materials, phenol-sulfuric acid method as the method of polysaccharide extraction rate, polysaccharide extraction rate as an indicator, the optimum process conditions for polysaccharide extraction from Bletilla striata were obtained including liquid-solid ratio of 15 mg/mL, extraction temperature 90 °C, extraction time of 90 h, sample immersion time 90 min, and extraction 2 times, achieving the corresponding polysaccharide yield of 48.38%. The antioxidant assays in vitro revealed that the polysaccharides from Bletilla striata exhibited a slight scavenging activity for 1,1-diphenyl-2- picrylhydrazyl radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cations.

关键词: Bletilla striata, polysaccharides, extraction, antioxidant activities

Abstract: In this paper, the extraction conditions of water-soluble polysaccharide from Bletilla striata were optimized by response surface methodology. With the dried Bletilla striata as raw materials, phenol-sulfuric acid method as the method of polysaccharide extraction rate, polysaccharide extraction rate as an indicator, the optimum process conditions for polysaccharide extraction from Bletilla striata were obtained including liquid-solid ratio of 15 mg/mL, extraction temperature 90 °C, extraction time of 90 h, sample immersion time 90 min, and extraction 2 times, achieving the corresponding polysaccharide yield of 48.38%. The antioxidant assays in vitro revealed that the polysaccharides from Bletilla striata exhibited a slight scavenging activity for 1,1-diphenyl-2- picrylhydrazyl radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cations.

Key words: Bletilla striata, polysaccharides, extraction, antioxidant activities

中图分类号:

Xiaojing Shena, Weiwei Jiang. Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro[J]. Medicine Research, 2020, 4(3): 200010-.

Xiaojing Shena , Weiwei Jiang. Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro[J]. Medicine Research, 2020, 4(3): 200010-.

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Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro

Polysaccharides from Bletilla striata: Extraction, Optimization and Their Antioxidant Activities in vitro

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