An Effective Inhibitory of Triptolide on Ovarian Cancer

doi:10.21127/yaoyimr20200017

Medicine Research ›› 2020, Vol. 4 ›› Issue (4): 200017-200017.DOI: 10.21127/yaoyimr20200017

所属专题： Anticancer Agents

An Effective Inhibitory of Triptolide on Ovarian Cancer

Xiaodi Li,^†,a,d Rui Liu,^†,c Ping Wang,^*,b,c and Jun Zhu^*,b,c

^aGraduate School, Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Shanghai 201203, China
^b Shanghai University of Medicine & Health Sciences, 279 Zhou Zhu Highway, Shanghai 201318, China
^c National Engineering Research Center for Nanotechnology, 28 East Jiang Chuan Road, Shanghai 200241, China
^d Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Shanghai 201203, China

收稿日期:2020-10-16 修回日期:2020-11-15 出版日期:2020-12-20 发布日期:2020-12-20
基金资助:

An Effective Inhibitory of Triptolide on Ovarian Cancer

Xiaodi Li,^†,a,d Rui Liu,^†,c Ping Wang,^*,b,c and Jun Zhu^*,b,c

^aGraduate School, Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Shanghai 201203, China
^b Shanghai University of Medicine & Health Sciences, 279 Zhou Zhu Highway, Shanghai 201318, China
^c National Engineering Research Center for Nanotechnology, 28 East Jiang Chuan Road, Shanghai 200241, China
^d Shanghai University of Traditional Chinese Medicine, 1200 Cai Lun Road, Shanghai 201203, China

Received:2020-10-16 Revised:2020-11-15 Online:2020-12-20 Published:2020-12-20
Contact: yzjzhu@163.com (J. Z.), pingw2006@163.com (P. W.).
†Xiaodi Li and Rui Liu contributed equally to this work.
Supported by:

摘要/Abstract

摘要： This study explores the inhibitory effect of triptolide on ovarian cancer cells through in vitro cell and in vivo animal model experiments. We use the CCK-8 survival rate experiment to determine appropriate concentration of triptolide and the type of cell line including SF9, HEK293, A2780 and Skov-3. Furthermore, the cell invasion and migration of A2780 and Skov-3 cells shows that triptolide had a strong inhibitory effect on ovarian cancer cell line. In addition, flow cytometry is used to detect the apoptosis and cycle of A2780 cells. The results prove that the proliferation of ovarian cancer cell lines is significantly inhibited by triptolide at a 200 nmol/L concentration. Finally, in vivo animal model shows a strong inhibitory effect of triptolide with a 200 nmol/L concentration on ovarian cancer. However, HE staining of animal liver, kidney and ovarian tissue section reveals that there is a stronger effect of 200 nmol/L triptolide on the damage of living organs coming from its toxicity. The result offers a chance for reasonable safety use of triptolide.

关键词: Triptolide, Ovarian cancer, Inhibitory, Toxicity

Abstract: This study explores the inhibitory effect of triptolide on ovarian cancer cells through in vitro cell and in vivo animal model experiments. We use the CCK-8 survival rate experiment to determine appropriate concentration of triptolide and the type of cell line including SF9, HEK293, A2780 and Skov-3. Furthermore, the cell invasion and migration of A2780 and Skov-3 cells shows that triptolide had a strong inhibitory effect on ovarian cancer cell line. In addition, flow cytometry is used to detect the apoptosis and cycle of A2780 cells. The results prove that the proliferation of ovarian cancer cell lines is significantly inhibited by triptolide at a 200 nmol/L concentration. Finally, in vivo animal model shows a strong inhibitory effect of triptolide with a 200 nmol/L concentration on ovarian cancer. However, HE staining of animal liver, kidney and ovarian tissue section reveals that there is a stronger effect of 200 nmol/L triptolide on the damage of living organs coming from its toxicity. The result offers a chance for reasonable safety use of triptolide.

Key words: Triptolide, Ovarian cancer, Inhibitory, Toxicity

中图分类号:

Xiaodi Li, Rui Liu, Ping Wang, and Jun Zhu. An Effective Inhibitory of Triptolide on Ovarian Cancer[J]. Medicine Research, 2020, 4(4): 200017-200017.

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An Effective Inhibitory of Triptolide on Ovarian Cancer

An Effective Inhibitory of Triptolide on Ovarian Cancer

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