Zeolitic Imidazolate Framework-8 (ZIF-8) is a type of typical metal organic framework (MOF), which is an emerging porous coordination polymers that is bridged by zinc metal ions and organic linkages. Due to its intrinsic porous characteristics, unique pH sensitivity as well as superior drug loading efficiency, zeolitic imidazolate framework-8 has attracted extensive attention and received a wide range of applications in the field of biomedicine. In this review, we briefly discuss the effective delivery and controlled release of various drugs in ZIF-8, such as functional proteins, hydrophilic and hydrophobic drugs. Understanding the processes of various drugs loading is important for developing new drug delivery systems. In addition, we also explored the cooperation mechanism between ZIF and different materials. The combination of chemotherapy, photodynamic therapy, photothermal therapy and medical imaging have been applied in the field of nanomedicine and have made emarkable achievements.

The solution structure and function of human coagulation factor X (FX) were studied to understand the key structural elements of the enzyme form during activation. FX is a vitamin K-dependent plasma glycoprotein, composed of light and heavy chains, and plays a central role in the coagulation cascade. The calcium-rich γ-carboxyglutamic acid domain is responsible for binding to the membrane, an important step in the coagulation process. After activation, the serine protease domain undergoes a significant relocation, forming a compact multidomain. The S1-specific pocket largely determines the functional activities that activate FX. The enzyme-producing form of FX is more extensive than the active form. The enzyme-producing and activated forms of FX have different conformations in the serine protease domain.

Chitosan is a kind of amino polysaccharide biopolymer with good film-forming, biocompatibility, biodegradability, bacteriostasis and nontoxic, obtained from the deacetylation of chitin, which contains abundant active amino and hydroxyl groups. With creative properties, functions and various applications especially in the biomedical field, chitosan is mainly used as pharmaceutical membrane, gel, microsphere (microcapsule), nanoparticle, drug and gene delivery, etc. This minireview provides an overview on the structure and properties of chitosan, the applications of chitosan biomaterials is introduced, and its research and development in the biomedical field are prospected.