Magnesium Ion-Dependent Activation of Glucokinase in Simulated Cytoplasmic System in Vitro

doi:10.21127/yaoyimr20230011

Medicine Research ›› 2023, Vol. 7 ›› Issue (3-4): 230011-230011.DOI: 10.21127/yaoyimr20230011

Magnesium Ion-Dependent Activation of Glucokinase in Simulated Cytoplasmic System in Vitro

Xinyi Pu^a, Boyu Chen^a, Xiaozhen Diao^a,b, Hui Han^a, Jinhua Hou^a, Jiudong Hu^c, Yuhuan Yang^a, Yuping Zhu^d,*, Wenhui Wu^a,e,*

^aDepartment of Marine Biopharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;
^bInnovative Platform for Marine Biopharmaceutical Technology in Lingang New Area, Shanghai 201306, China;
^cShanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China;
^dCollege of Basic Medical Sciences, Navy Medical University, Shanghai 200433, China;
^eEast China Sea Marine Biological Resources Engineering Technology Center, Zhoushan, Zhejiang 316100, China

Received:2023-11-01 Accepted:2023-12-12 Online:2023-12-20 Published:2024-02-22
Contact: * Email: ypz@smmu.edu.cn (Y. Z.); whwu@shou.edu.cn (W. W.)

Abstract

Abstract: Glucokinase (GK), as a key enzyme regulating carbohydrate metabolism, has been widely researched as a therapeutic target for diabetes. This study aimed to investigate the influence of magnesium ion concentration, ATP concentration, and enzyme concentration on the catalytic activity of GK by response surface. A cellular model of insulin resistance (IR) was established to observe the ameliorating effect of magnesium ion on IR by screening the concentration and duration of insulin action. The results indicated that the optimal concentrations for enzyme activity were found to be 1.1 mM for magnesium ion, 1.4 mM for ATP, and 0.5 U/mL for glucokinase. In the presence of taurine, the IR model was treated with magnesium ion (1.1 mM) can promote glucose consumption, GK activity and intracellular magnesium concentration in IR cells. Magnesium ion, as a key factor in GK-catalyzed glycolysis, showed a positive correlation between intracellular magnesium ions and the catalytic activity of GK within a certain concentration range. Therefore, magnesium ion could effectively promote carbohydrate metabolism and alleviate the progression of IR.

Key words: glucokinase, magnesium ion, response surface, intracellular, insulin resistance

Xinyi Pu, Boyu Chen, Xiaozhen Diao, Hui Han, Jinhua Hou, Jiudong Hu, Yuhuan Yang, Yuping Zhu, Wenhui Wu. Magnesium Ion-Dependent Activation of Glucokinase in Simulated Cytoplasmic System in Vitro[J]. Medicine Research, 2023, 7(3-4): 230011-230011.

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Magnesium Ion-Dependent Activation of Glucokinase in Simulated Cytoplasmic System in Vitro

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